Simultaneous high-throughput recombinational cloning of open reading frames in closed and open configurations
نویسندگان
چکیده
منابع مشابه
Simultaneous cloning of open reading frames into several different expression vectors.
Genomic sequencing has enabled the prediction of thousands of genes, most of which either cannot be assigned a function or can be only broadly categorized on the basis of sequence alone. High-throughput strategies for elucidating protein function are of high priority, and numerous approaches are being developed. Many of these approaches require the cloning of open reading frames (ORFs) into exp...
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A plasmid was constructed that facilitates the cloning and expression of open reading frame DNA. A DNA fragment containing a bacterial promoter and the amino terminus of the cI gene of bacteriophage lambda was fused to an amino-terminally deleted version of the lacZ gene. An appropriate cloning site was inserted between these two fragments such that a frameshift mutation was introduced upstream...
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Department of Environmental and Biomolecular Systems, Oregon Health and Science University, Beaverton, Oregon 97006, USA; Department of Molecular Microbiology and Immunology, Oregon Health and Science University, Portland, Oregon 97201, USA; Divisions of Human Biology and Clinical Research, Fred Hutchinson Cancer Research Center, Seattle, Washington 98109, USA; Department of Microbiology and De...
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In this paper the duties of an anesthesiologist and his right of i=;electing patient is discussed. La,ter, we di:=:cuss the <::mergencies cf heart surgery, pre up visit and alsc few dcails of pre up medication cf thei,e pati<mts. We also touch on the stages of anesthPsia and point to our preference for the use cf artery and vein for arterial and venous cannulation. In this paper we discuss in b...
متن کاملGenome-scale cloning and expression of individual open reading frames using topoisomerase I-mediated ligation.
The in vitro cloning of DNA molecules traditionally uses PCR amplification or site-specific restriction endonucleases to generate linear DNA inserts with defined termini and requires DNA ligase to covalently join those inserts to vectors with the corresponding ends. We have used the properties of Vaccinia DNA topoisomerase I to develop a ligase-free technology for the covalent joining of DNA fr...
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ژورنال
عنوان ژورنال: Plant Biotechnology Journal
سال: 2006
ISSN: 1467-7644,1467-7652
DOI: 10.1111/j.1467-7652.2006.00183.x